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adeno-associated virus (aav) backbones  (Cell Biolabs Inc)

 
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    Cell Biolabs Inc adeno-associated virus (aav) backbones
    Adeno Associated Virus (Aav) Backbones, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adeno-associated+virus+backbones/pm34725955-151-4-13?v=Cell+Biolabs+Inc
    Average 90 stars, based on 1 article reviews
    adeno-associated virus (aav) backbones - by Bioz Stars, 2026-07
    90/100 stars

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    FIG. 2. CRISPR-CLONInG: Replacement of partial cargo sequence (Cre-comp) with the desired donor sequence on adeno-associated virus (AAV) vector (#60229; Addgene). (A) Schematic illustration of AAV vector with CRISPR cut sites (red scissors) at two ends of the Cre-comp segment. Guides (AAV-A and AAV-B) with high on-target scores were selected. (B) Cre-comp was cut out with ctRNP (Cas9-ctRNA) complex; donor for gene replacement (containing 15 bp AA replacement sequence, noted as ‘‘R,’’ sandwiched by HA) flanked with complementary Gibson overhangs of the adjacent AAV backbone was PCR-amplified from custom gene synthesized plasmid. (C) Assembled AAV-v1: donor template cloned into the customized AAV backbone via Gibson (HiFi) Assembly. (D) Excised AAV vector backbone (*3.63 kb) and Cre-comp (*2.73 kb; left); PCR amplified donor template (*0.8 kb) with Gibson overhangs (right). (E) After CRISPR-CLONInG, 15/16 clones showed correct vector assembly, confirmed by BbsI RE(s) diagnosis (two DNA fragments; black arrow); three clones further validated by Sanger sequencing. Resolved on 0.9% agarose gel.

    Journal: The CRISPR journal

    Article Title: New Additions to the CRISPR Toolbox: CRISPR- CLONInG and CRISPR- CLIP for Donor Construction in Genome Editing.

    doi: 10.1089/crispr.2019.0062

    Figure Lengend Snippet: FIG. 2. CRISPR-CLONInG: Replacement of partial cargo sequence (Cre-comp) with the desired donor sequence on adeno-associated virus (AAV) vector (#60229; Addgene). (A) Schematic illustration of AAV vector with CRISPR cut sites (red scissors) at two ends of the Cre-comp segment. Guides (AAV-A and AAV-B) with high on-target scores were selected. (B) Cre-comp was cut out with ctRNP (Cas9-ctRNA) complex; donor for gene replacement (containing 15 bp AA replacement sequence, noted as ‘‘R,’’ sandwiched by HA) flanked with complementary Gibson overhangs of the adjacent AAV backbone was PCR-amplified from custom gene synthesized plasmid. (C) Assembled AAV-v1: donor template cloned into the customized AAV backbone via Gibson (HiFi) Assembly. (D) Excised AAV vector backbone (*3.63 kb) and Cre-comp (*2.73 kb; left); PCR amplified donor template (*0.8 kb) with Gibson overhangs (right). (E) After CRISPR-CLONInG, 15/16 clones showed correct vector assembly, confirmed by BbsI RE(s) diagnosis (two DNA fragments; black arrow); three clones further validated by Sanger sequencing. Resolved on 0.9% agarose gel.

    Article Snippet: CRISPR-CLONInG: Replacement of partial cargo sequence (Cre-comp) with the desired donor sequence on adeno-associated virus (AAV) vector (#60229; Addgene). (A) Schematic illustration of AAV vector with CRISPR cut sites (red scissors) at two ends of the Cre-comp segment.

    Techniques: CRISPR, Cloning, Sequencing, Virus, Plasmid Preparation, Synthesized, Clone Assay, Biomarker Discovery, Agarose Gel Electrophoresis